Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 465, Issue 2, Pages 389-398Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2007.06.035
Keywords
TRP channel; walker A motif; steady-state fluorescence; TNP-ATP; FITC
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Transient receptor potential channel vanilloid receptor subunit 1 (TRPV1) is a thermosensitive cation channel activated by noxious heat as well as a wide range of chemical stimuli. Although ATP by itself does not directly activate TRPV1, it was shown that intracellular ATP increases its activity by directly interacting with the Walker A motif residing on the C-terminus of TRPV1. In order to identify the amino acid residues that are essential for the binding of ATP to the TRPV1 channel, we performed the following point mutations of the Walker A motif: P732A, D733A, G734A, K735A, D736A, and D737A. Employing bulk fluorescence measurements, namely a TNP-ATP competition assay and FITC labelling and quenching experiments, we identified the key role of the K735 residue in the binding of the nucleotide. Experimental data was interpreted according to our molecular modelling simulations. (C) 2007 Elsevier Inc. All rights reserved.
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