Journal
FEBS LETTERS
Volume 581, Issue 23, Pages 4468-4472Publisher
WILEY
DOI: 10.1016/j.febslet.2007.08.029
Keywords
virus; glycoproteins; corneal fibroblasts; heparan sulfate; polykaryocytes
Funding
- NEI NIH HHS [EY03890, P30 EY001792, R01 EY003890, P30 EY01792] Funding Source: Medline
- NIAID NIH HHS [R01 AI057860, R01 AI057860-03] Funding Source: Medline
- PHS HHS [R01 AL057860] Funding Source: Medline
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One way herpes simplex virus type-1 (HSV-1) spreads in vivo is by polykaryocytes formation. Here we demonstrate that polykaryocyte production during HSV-1 spread in cultured human corneal fibroblasts (CF) required heparan sulfate (HS) and more specifically 3-O sulfated HS (3-OS HS). The polykaryocyte formation heavily depended on the expression of HS on target (CF) cells but not on glycoprotein expressing effector cells. Furthermore, we provide the first visual evidence of 3-OS HS and HSV-1 gD colocalization during the membrane fusion process. Taken together our results provide novel insight into the significance of HS in polykaryocyte formation. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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