Journal
SCIENCE
Volume 317, Issue 5845, Pages 1767-1770Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1146304
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- NHLBI NIH HHS [HL56949, HL068130, HL63143] Funding Source: Medline
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Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.
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