Journal
JOURNAL OF CELL BIOLOGY
Volume 178, Issue 7, Pages 1251-1264Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200703092
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- NIGMS NIH HHS [R01 GM042759, R01 GM050399, R37 GM042759, R01 GM50399, R01 GM42759] Funding Source: Medline
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Cofilin is the major mediator of actin. lament turnover in vivo. However, the molecular mechanism of cofilin recruitment to actin networks during dynamic actin-mediated processes in living cells and cofilin's precise in vivo functions have not been determined. In this study, we analyzed the dynamics of fluorescently tagged cofilin and the role of cofilin-mediated actin turnover during endocytosis in Saccharomyces cerevisiae. In living cells, cofilin is not necessary for actin assembly on endocytic membranes but is recruited to molecularly aged adenosine diphosphate actin. laments and is necessary for their rapid disassembly. Defects in cofilin function alter the morphology of actin networks in vivo and reduce the rate of actin flux through actin networks. The consequences of decreasing actin flux are manifested by decreased but not blocked endocytic internalization at the plasma membrane and defects in late steps of membrane trafficking to the vacuole. These results suggest that cofilin-mediated actin filament flux is required for the multiple steps of endocytic trafficking.
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