Both TRIM5α and TRIMCyp have only weak antiviral activity in canine D17 cells

Background: TRIM5 alpha, which is expressed in most primates and the related TRIMCyp, which has been found in one of the New World monkey species, are antiviral proteins of the TRIM5 family that are able to intercept incoming retroviruses early after their entry into cells. The mechanism of action has been partially elucidated for TRIM5a, which seems to promote premature decapsidation of the restricted retroviruses. In addition, through its N-terminal RING domain, TRIM5 alpha may sensitize retroviruses to proteasome-mediated degradation. TRIM5 alpha-mediated restriction requires a physical interaction with the capsid protein of targeted retroviruses. It is unclear whether other cellular proteins are involved in the inhibition mediated by TRIM5 alpha and TRIMCyp. A previous report suggested that the inhibition of HIV-I by the rhesus macaque orthologue of TRIM5 alpha was inefficient in the D17a canine cell line, suggesting that the cellular environment was important for the restriction mechanism. Here we investigated further the behavior of TRIM5 alpha and TRIMCyp in the D17 cells. Results: We found that the various TRIM5 alpha orthologues studied (human, rhesus macaque, African green monkey) as well as TRIMCyp had poor antiviral activity in the D17 cells, despite seemingly normal expression levels and subcellular distribution. Restriction of bothHIV- 1 and the distantly related N-tropic murine leukemia virus (N-MLV) was low in D17 cells. Both TRIM5 alpha rh and TRIMCyp promoted early HIV-I decapsidation in murine cells, but weak levels of restriction in D17 cells correlated with the absence of accelerated decapsidation in these cells and also correlated with normal levels of cDNA synthesis. Fv1, a murine restriction factor structurally unrelated to TRIM5 alpha, was fully functional in D17 cells, showing that the loss of activity was specific to TRIM5 alpha/TRIMCyp. Conclusion: We show that D17 cells provide a poor environment for the inhibition of retroviral replication by proteins of the TRIM5 family. Because both TRIM5a and TRIMCyp are poorly active in these cells, despite having quite different viral target recognition domains, we conclude that a step either upstream or downstream of target recognition is impaired. We speculate that an unknown factor required for TRIM5 alpha and TRIMCyp activity is missing or inadequately expressed in D17 cells.