Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1166, Issue 1-2, Pages 142-151Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.08.028
Keywords
glucosinolates; Isatis tinctoria; Brassicaceae; pressurized liquid extraction (PLE); accelerated solvent extraction (ASE); quantitative analysis; quantitative; NMR; LC-MS; ion-pair chromatography; broccoli; mustard; garden cress
Ask authors/readers for more resources
Glucosinolates have attracted significant interest due to the chernopreventive properties of some of their transformation products. Numerous protocols for the extraction and analysis of glucosinolates have been published, but limited effort has been devoted to optimize and validate crucial extraction parameters and sample preparation steps. We carried out a systematic optimization and validation of a quantitative assay for the direct analysis of intact glucosinolates in Isatis tinctoria leaves (woad, Brassicaceae). Various parameters such as solvent composition, particle size, temperature, and number of required extraction steps were optimized using pressurized liquid extraction (PLE). We observed thermal degradation of glucosinolates at temperatures above 50 degrees C, and loss of > 60% within 10min at 100 degrees C, but no enzymatic degradation in the leaf samples at ambient temperature. Excellent peak shape and resolution was obtained by reversed-phase chromatography on a Phenomenex Aqua column using 10 mM ammonium formate as ion-pair reagent. Detection was carried out by electrospray ionisation mass spectrometry in the negative ion mode. Analysis of cruciferous vegetables and spices such as broccoli (Brassica oleracea L. var. italica), garden cress (Lepidium sativum L.) and black mustard (Sinapis nigra L.) demonstrated the general applicability of the method. (c) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available