Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 19, Pages 7416-7423Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac071460e
Keywords
-
Categories
Ask authors/readers for more resources
Mass spectrometry imaging (MSI) acquires a grid of spatially resolved mass spectra and provides a molecular landscape of a tissue. This can have a myriad of uses: from basic tissue characterization to a comprehensive pathological diagnosis. We have developed a fast, inexpensive, histology-compatible tissue preparation method for matrix-assisted laser desorption/ionization (MALDI)MSI, which overcomes current sample preparationimposed limitations in image resolution. Tissue sections are prepared via simultaneous fixation and matrix deposition. This is accomplished by incorporating the MALDI matrix into solvents that preserve tissue integrity when applied according to standard histology procedures. This concept was expanded to include multiple histology protocols, thereby enabling analysis to be tailored to a variety of biomolecules and tissue types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available