Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 36, Issue 2, Pages 132-145Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2007.05.013
Keywords
patch clamp; T-type Ca2+ channel; absence epilepsy; thalamus; real time PCR
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It has been suggested that the specific burst firing patterns of thalamic neurons reflect differential expression of low-voltage-activated (LVA) Ca2+ channel subtypes and their splice variants. By combining electrophysiological, molecular biological, immunological, and computational modeling techniques we here show that diverging LVA Ca2+ currents of thalamocortical relay (TC) and GABAergic interneurons of the dLGN correlate with a differential expression of LVA Ca2+ channel splice variations and isoforms (alpha 1G-a in TC; alpha 1G-bc and alpha 1I in interneurons). Implementation of the observed LVA Ca2+ current differences into a TC neuron model changed the burst firing from TC-like to interneuron-like. We conclude that alternative splicing of the alpha 1G isoform in dLGN TC and interneurons, and the exclusive expression of the alpha 1I isoform in interneurons play a prominent role in setting the different LVA Ca2+ current properties of TC and interneurons, which critically contribute to the diverging burst firing behavior of these neurons. (c) 2007 Elsevier Inc. All rights reserved.
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