Journal
MOLECULAR AND CELLULAR PROBES
Volume 21, Issue 5-6, Pages 408-411Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2007.06.008
Keywords
cytochrome P450 (CYP450); pharmacogenetic testing; SimpleProbe probe; TaqMan probe
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Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan((R)) and LightCycler (TM) for the pharmacogenctic evaluation of CYP2B6 516G > T polymorphism. Both methods were found to be suitable for pharmacogenetic testing of CYP2B6 516G > T in the meaning of time consumption, accurate genotype calling, flexible reaction format, simple data analysis, and low cost per assay. The genotype success rate was similar, but the LightCycler procedure was less expensive in terms of cost per sample and shorter running time. (c) 2007 Elsevier Ltd. All rights reserved.
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