Journal
CELL BIOLOGY INTERNATIONAL
Volume 31, Issue 10, Pages 1225-1230Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cellbi.2007.04.006
Keywords
transforming growth factor-beta; epithelial-mesenchymal transition; Smad; Rho; ROCK
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Funding
- Korea Health Promotion Institute [0420050] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Lens epithelial cells undergo epithelial-mesenchymal transition (EMT) after injury as in cataract extraction, leading to fibrosis of the lens capsule. We have recently shown that TGF-beta-induced EMT in lens epithelial cells depends on PI3 kinase/Akt signal pathway. In this report, we suggest Smad3 is necessary for TGF-beta-induced EMT by showing that the expression of dominant-negative Smad3 blocks the expression of a-smooth muscle actin (alpha-SMA) and morphological changes. We also show that TGF-induces a biphasic change in Rho activity, and that Y27632, a selective inhibitor of Rho effector ROCK, inhibits TGF-beta-induced EMT in vitro and in vivo. We finally show that Smad3 activation and Rho signal activation is independent each other. All of these findings suggest that Rho/ROCK activation together with Smad3 is necessary for TGF-beta-induced EMT in lens epitheliat cells. (c) 2007 International Federation for Cell Biology. (c) Published by Elsevier Ltd. All rights reserved.
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