Journal
MOLECULAR MICROBIOLOGY
Volume 66, Issue 1, Pages 206-219Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2958.2007.05906.x
Keywords
-
Categories
Funding
- NIAID NIH HHS [R01 AI057637] Funding Source: Medline
- NIDDK NIH HHS [T32 DK007542] Funding Source: Medline
Ask authors/readers for more resources
CodY, a global regulator of gene expression in low G + C Gram-positive bacteria, was found to repress toxin gene expression in Clostridium difficile. Inactivation of the codYgene resulted in derepression of all five genes of the C. difficile pathogenicity locus during exponential growth and stationary phase. CodY was found to bind with high affinity to a DNA fragment containing the promoter region of the tcdR gene, which encodes a sigma factor that permits RNA polymerase to recognize promoters of the two major toxin genes as well as its own promoter. CodY also bound, but with low affinity, to the toxin gene promoters, suggesting that the regulation of toxin gene expression by CodY occurs primarily through direct control of tcdR gene expression. Binding of CodY to the tcdR promoter region was enhanced in the presence of GTP and branched-chain amino acids, suggesting a link between nutrient limitation and the expression of C. difficile toxin genes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available