Journal
TRAFFIC
Volume 8, Issue 10, Pages 1304-1312Publisher
WILEY
DOI: 10.1111/j.1600-0854.2007.00617.x
Keywords
apical; basolateral; Caco-2; electroporation; MDCK; polarized epithelium; RPE
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Funding
- NEI NIH HHS [R01 EY008538, EY08538] Funding Source: Medline
- NIGMS NIH HHS [R01 GM034107, GM34107] Funding Source: Medline
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Electroporation-mediated delivery of molecules is a procedure widely used for transfecting complementary DNA in bacteria, mammalian and plant cells. This technique has proven very efficient for the introduction of macromolecules into cells in suspension culture and even into cells in their native tissue environment, e.g. retina and embryonic tissues. However, in spite of several attempts to date, there are no well-established procedures to electroporate polarized epithelial cells adhering to a tissue culture substrate (glass, plastic or filter). We report here the development of a simple procedure that uses available commercial equipment and works efficiently and reproducibly for a variety of epithelial cell lines in culture.
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