Journal
LAB ANIMAL
Volume 36, Issue 9, Pages 43-50Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/laban1007-43
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Though pinworm infestation has been prevalent since the early years of Laboratory animal medicine, the genomes of these parasites have not yet been sequenced. The authors used high-fidelity polymerase chain reaction to amplify a large portion of the ribosomal gene complex of four pinworm species commonly found in lab rodents and rabbits (Aspiculuris tetraptero, Passalurus ambiguus, Syphacia muris and Syphacia obvelato). They determined DNA sequences for these complexes and carried out phylogenetic analysis. Using this information, the authors developed real-time molecular beacon assays for pinworm detection, comparing the new diagnostic approach with traditional methods such as perianal tape testing, fecal flotation and direct examination of intestinal content.
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