Journal
DNA REPAIR
Volume 6, Issue 10, Pages 1529-1535Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2007.04.007
Keywords
gene repair; single-stranded oligonucleotides
Categories
Funding
- NCI NIH HHS [R01 CA089325, R01 CA089325-05A2, R01 CA089325-06, R01 CA 89325-01A1] Funding Source: Medline
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We have previously shown that activation of the homologous recombinational repair pathway leads to a block of cell division in corrected cells, possibly through the activity of checkpoint proteins Chk1 and Chk2. In this study, we examine the long-term impact of this stalling on the growth of cells that have enabled gene repair events. Using a mutated eGFP gene as an episomal. reporter, we show that corrected (eGFP-positive) cells contain only a few active replication templates 2 weeks after electroporation, yet do not display an apoptotic or senescent phenotype. By 6 weeks after electroporation, cells resume active replication with a cell cycle profile that is comparable to that of the non-corrected (eGFP-negative) population. These results indicate that the initial stalling is transient and eGFP-positive cells eventually resume a normal phenotypic growth pattern, allowing for passaging and expansion in Vitro (C) 2007 Elsevier B.V. All rights reserved.
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