Journal
ELECTROPHORESIS
Volume 28, Issue 20, Pages 3786-3793Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200700215
Keywords
dynamic pH junction; online sample preconcentration; pH-mediated stacking
Funding
- NCI NIH HHS [T32 CA009242, T32CA09242] Funding Source: Medline
- NIBIB NIH HHS [R01EB00247, R01 EB000247, R01 EB000247-17] Funding Source: Medline
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A technique has been developed to enhance analyte focusing for CE for the analysis of physiological samples. High-ionic-strength samples are titrated to low-ionic-strength online using pH-mediated sample stacking in conjunction with a dynamic pH junction. This method concentrates analytes by reducing their electrophoretic mobility during field-amplification. Parameters responsible for enhanced focusing were investigated, and an enhanced pH-mediated stacking method was optimized for anionic nucleosides. The process results in ultra-narrow peak widths, for example, 0.28 s for thymidine with a 10 min analysis time. Peak width and resolution with the enhanced stacking method were also compared to normal base stacking and electrokinetic injection. With this technique, mass-loading capacity can be increased without degradation in peak shape and resolution is dramatically improved.
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