Pro-inflammatory cellular immune response in Behcet's disease

Several proteins are investigated as candidate auto-antigens in the pathogenesis of Behcet's disease ( BD). This study aimed to screen the cellular responses to autoantigen (alpha B-crystallin, alpha BC), and microorganism ( Streptococcus sangius KTH-1 BES-1 protein) derived peptides as well as to isopentenyl pyrophosphate ( IPP). Peripheral blood mononuclear cells ( PBMC) from 22 BD patients and 22 healthy controls ( HC) were stimulated in vitro with alpha BC, BES-1 peptides, IPP and PPD and induced proliferation as well as the secreted interleukin ( IL)-12 and interferon ( IF)-gamma production levels were measured. We did not observe any significant difference in cellular proliferation between BD patients and HC. Induction of IL-12 secretion with alpha BC was stronger in BD patients ( P = 0.04) and in the disease subgroup with uveitis ( P = 0.027) compared the HC. When responses to PPD were compared, proliferation of PMBC was lower ( P = 0.03), whereas IL-12 secretion was higher in BD ( P = 0.04) as well as in patients under colchicum treatment ( P = 0.04) and with vascular involvement ( P = 0.006) compared to HC. BES-1(373-385) peptide induced also higher IL-12 productions by PBMC of BD patients ( P = 0.017) and of patients with uveitis ( P = 0.013). Finally, IPP stimulated higher IL-12 secretions from PBMC in BD patients ( P = 0.035) and in patients with ( P = 0.02) or without ( P = 0.017) uveitis or arthritis ( P = 0.04), under colchicum treatment ( P = 0.01) or not receiving any immunosuppressive treatment ( P = 0.007) compared to HC. These results suggest a more prominent pro-inflammatory cytokine secretion from PBMC in BD patients compared to HC in response to various antigens including alpha BC protein, BES-1(373-385), IPP and PPD.