Journal
INTERNATIONAL IMMUNOLOGY
Volume 19, Issue 10, Pages 1165-1173Publisher
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxm086
Keywords
bioluminescence; luciferase
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Funding
- NIAID NIH HHS [AI035783, AI056356, AI057998] Funding Source: Medline
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After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and revealed several unexpected features. First, the T cells localized to the site of vector injection, as well as the draining lymph node within 24-48 h. Second, the major site to which T cells later redistributed were intra-abdominal lymphoid organs, including milky spots, mesenteric and lumbar lymph nodes. We determined the relationship between bioluminescent signal and antigen-specific T cell numbers in various lymphoid organs, and concluded that bioluminescent signal is a valid surrogate measure of T cell abundance in superficial lymph nodes, but not in deeper structures such as the spleen.
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