Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 82, Issue 4, Pages 1003-1010Publisher
WILEY
DOI: 10.1189/jlb.0207103
Keywords
mast cells; Fc receptors; allergy
Categories
Funding
- NIAID NIH HHS [AI070345, AI20253] Funding Source: Medline
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Previous studies about basophils and mast cells identified the ability of IgE to up-regulate Fc epsilon RI expression by a process that depends on stabilization of the surface receptor by IgE. However, the mechanism of loss from the cell surface, when unoccupied, is not known. The current studies have examined whether unoccupied Fc epsilon RI on basophils is lost by shedding or endocytosis. IgE was dissociated partially from purified human basophils to augment loss of the unoccupied receptor, and comparisons were made between basophils +/- IgE resensitization prior to 1-day culture. Incubation did not result in a detectable receptor in culture supernatants. However, in the presence of IL-3, although total cell surface expression decreased by 30% (relative to resensitized cells), Fc epsilon RI from whole cell lysates was not statistically different between the two conditions. Incubation for 18 h without IL-3 resulted in the same loss from the cell surface but equivalent loss in whole cell lysates. This degradation process was reversible with Bafilomycin A. There was also evidence that the internalized receptor could be recycled. After the initial 18-h down-regulation, the receptor could be found partially restored to the cell surface if IgE were added back to the culture +/- cycloheximide. Loss of the unoccupied receptor, as well as accumulation of the receptor under the influence of IgE, was found to be insensitive to the presence of a src-family kinase inhibitor, PP1. These studies establish that the unoccupied receptor is lost by a process of endocytosis, partially recycled to the cell surface, and ultimately degraded by a lysosomal mechanism.
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