Journal
JOURNAL OF SURGICAL RESEARCH
Volume 142, Issue 2, Pages 301-303Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2007.03.043
Keywords
Bergstrom muscle biopsy needle; wall suction enhancement technique; muscle biopsies; skeletal muscle; vastus lateralis; tissue sample size
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Funding
- NCRR NIH HHS [M01 RR10710] Funding Source: Medline
- NIA NIH HHS [R01 AG17446-01A2] Funding Source: Medline
- NIDDK NIH HHS [R01 DK049316-09A1] Funding Source: Medline
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Background. The needle biopsy technique described by Bergstrom is the most commonly used technique to obtain samples to assess muscle metabolism. Sampling of muscle, particularly the vastus lateralis, has become an essential tool in biomedical and clinical research. Optimal sample size is critical for availability of tissue for processing. To evaluate the effectiveness of a novel technique to obtain adequate sample size using wall suction applied to needle muscle biopsy, we collected samples from subjects in on-going clinical studies for gene expression. Materials and methods. Muscle biopsy samples of the vastus lateralis using 6 mm Bergstrom needles under local anesthesia were obtained from 55 subjects who had volunteered to participate in this research project. The vastus lateralis was biopsied according to the methods described by Bergstrom with a 6 mm biopsy needle. Wall suction was applied to the inner bore of the biopsy needle after the needle was inserted into the muscle. Results. The mean sample of biopsy taken using the 6 nun was 233 mg (n = 55). The wall suction (200 mm Hg) applied to the needle pulled the surrounding tissue into the central bore of the needle. The quality of the samples was adequate for all biochemical assays. The biopsy technique did not result in any complications due to infection or bleeding. Conclusions. Using a novel technique of connecting a 6 mm Bergstrom biopsy needle to wall suction, we have obtained 200 to 300 mg muscle biopsy specimens uniformly, with ease, and minimal discomfort. An increase in sample size allows for a wider variety of biochemical and histopathological analysis. (C) 2007 Elsevier Inc. All rights reserved.
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