Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 68, Issue 2, Pages 252-262Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2007.06.002
Keywords
bioslurry phase reactor; chloroyrifos; soil; bioremediation; Taguchi approach; design of experiments (DOE); orthogonal array (OA); optimization
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Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was applied to evaluate the influence of eight biotic and abiotic factors (substrate-loading rate, slurry phase pH, slurry phase dissolved oxygen (DO), soil water ratio, temperature, soil microflora load, application of bioaugmentation and humic substance concentration) on the soil bound chlorpyrifos bioremediation in bioslurry phase reactor. The selected eight factors were considered at three levels (18 experiments) in the experimental design. Substrateloading rate showed significant influence on the bioremediation process among the selected factors. Derived optimum operating conditions obtained by the methodology showed enhanced chlorpyrifos degradation from 1479.99 to 2458.33 mu g/g (over all 39.82% enhancement). The proposed method facilitated systematic mathematical approach to understand the complex bioremediation process and the optimization of near optimum design parameters, only with a few well-defined experimental sets. (C) 2007 Elsevier Inc. All rights reserved.
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