Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 39, Issue 2, Pages 133-139Publisher
SPRINGER
DOI: 10.1007/s10858-007-9180-8
Keywords
nonuniform sampling; maximum entropy reconstruction; reduced dimensionality; sparse sampling; multidimensional NMR
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Funding
- NCRR NIH HHS [RR-20125] Funding Source: Medline
- NIBIB NIH HHS [EB-001496] Funding Source: Medline
- NIGMS NIH HHS [GM-72000, GM-47467, P01 GM047467] Funding Source: Medline
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Developments in superconducting magnets, cryogenic probes, isotope labeling strategies, and sophisticated pulse sequences together have enabled the application, in principle, of high-resolution NMR spectroscopy to biomolecular systems approaching 1 megadalton. In practice, however, conventional approaches to NMR that utilize the fast Fourier transform, which require data collected at uniform time intervals, result in prohibitively lengthy data collection times in order to achieve the full resolution afforded by high field magnets. A variety of approaches that involve nonuniform sampling have been proposed, each utilizing a non-Fourier method of spectrum analysis. A very general non-Fourier method that is capable of utilizing data collected using any of the proposed nonuniform sampling strategies is maximum entropy reconstruction. A limiting factor in the adoption of maximum entropy reconstruction in NMR has been the need to specify non-intuitive parameters. Here we describe a fully automated system for maximum entropy reconstruction that requires no user-specified parameters. A web-accessible script generator provides the user interface to the system.
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