Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 20, Pages 6444-6449Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01425-07
Keywords
-
Categories
Ask authors/readers for more resources
We have determined the functions of the enzymes encoded by the lnpB, InpC, and InpD genes, located downstream of the lacto-N-biose phosphorylase gene (lnpA), in Bifidobacterium longum JCM1217. The lnpB gene encodes a novel kinase, N-acetylhexosamine 1-kinase, which produces N-acetylhexosamine I-phosphate; the InpC gene encodes UDP-glucose hexose 1-phosphate uridylyltransferase, which is also active on N-acetylhexosamine I-phosphate; and the lnpD gene encodes a UDP-glucose 4-epimerase, which is active on both UDPgalactose and UDP-N-acetylgalactosamine. These results suggest that the gene operon lnpABCD encodes a previously undescribed lacto-N-biose I/galacto-N-biose metabolic pathway that is involved in the intestinal colonization of bifidobacteria and that utilizes lacto-N-biose I from human milk oligosaccharides or galacto-N-biose from mucin sugars.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available