Journal
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
Volume 59, Issue 5, Pages 1344-1347Publisher
ARQUIVO BRASILEIRO MEDICINA VETERINARIA ZOOTECNIA
DOI: 10.1590/S0102-09352007000500040
Keywords
japanse quail; ELISA; IgG; newcastle disease
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An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation.
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