Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 71, Issue 1, Pages 75-77Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2007.06.021
Keywords
Cyclospora cayetanensis; detachment solution; flow cytometry; renocal-sucrose gradient
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Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry. Published by Elsevier B.V.
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