Journal
PARASITE IMMUNOLOGY
Volume 29, Issue 10, Pages 525-533Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-3024.2007.00971.x
Keywords
Alhydrogel; aluminium hydroxide; Montanide ISA 720; Plasmodium vivax; Pvs25H; rhesus
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Funding
- Intramural NIH HHS Funding Source: Medline
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Pvs25 is an ookinete surface protein from Plasmodium vivax that is the target of transmission-blocking antibodies. Two immunogenicity trials in rhesus monkeys with a recombinant form of the protein, Pvs25H, were undertaken. Monkeys were vaccinated with Pvs25H adsorbed to Alhydrogel or emulsified in Montanide ISA 720 at 0, 4 and 27 weeks (study 1) or in Montanide ISA 720 at 0 and 18 weeks (study 2) with 1.5 or 15 mu g Pvs25H in 0.1 or 0.5 mL of emulsion (four combinations). Immunogenicity was assessed by ELISA and by membrane-feeding experiments using P. vivax-infected blood from human volunteers (studies 1 and 2) or from chimpanzees (study 1). Both vaccine trials generated antibodies that blocked transmission of P. vivax to mosquitoes. Antibody titres and transmission blocking were higher with Montanide ISA 720 than with Alhydrogel in the first trial and with the 15 mu g Pvs25H/0.5 mL ISA 720 combination in the second trial.
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