Journal
JOURNAL OF ORTHOPAEDIC RESEARCH
Volume 25, Issue 10, Pages 1333-1342Publisher
WILEY
DOI: 10.1002/jor.20411
Keywords
OP-1; bone marrow cells; connective tissue progenitors; bone graft; femur defect
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Funding
- NIAMS NIH HHS [R01 AR042997, R01 AR42997] Funding Source: Medline
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Osteogenic Protein-1 (OP-1, BMP-7) acts locally on connective tissue progenitors (CTPs) to induce bone formation. The response to OP-1 and similar agents is potentially limited by the number of local CTPs. This study tested the hypothesis that supplementing local CTPs using autogenous bone marrow will enhance bone formation at an OP-1 implant. Four 1.0-cm diameter unicortical cylindrical defects in the left proximal femur were grafted in each of seven dogs. Radial ingrowth of new bone formation was assessed at 4 weeks using micro CT. The OP-1(3.5 mg rhOP-1 in 1 g bovine collagen I matrix) was implanted in each site combined with either clotted blood or aspirated bone marrow (BM). Bone formation was increased in the group augmented with transplanted marrow. These data suggest that increasing the local population of cells and CTPs using aspirated bone marrow can enhance the performance of OP-1, but may not eliminate the effects of site variation on the response to OP-1 and similar agents. The canine multiple femoral defect model defined in this study is well suited to quantitatively evaluate strategies for augmenting bone repair using local cell targeting and cell transplantation strategies. (c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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