A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells

A modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5 +/- 0.9%, while that of the particle bombardment method is at the range 2.0 +/- 0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.