Journal
BIOLOGIA
Volume 62, Issue 5, Pages 529-532Publisher
SPRINGER
DOI: 10.2478/s11756-007-0104-6
Keywords
Agrobacterium-mediated transformation; intrinsically fluorescent protein localization; onion epidermal cell
Categories
Ask authors/readers for more resources
A modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5 +/- 0.9%, while that of the particle bombardment method is at the range 2.0 +/- 0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available