Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 40, Issue 2, Pages 129-134Publisher
ELSEVIER
DOI: 10.1016/j.jcv.2007.07.007
Keywords
varicella-zoster virus; invader plus (R) method; Oka varicella vaccine
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Wereport the use of aprototype Invader Plus (R) method (Third Wave Technologies, Inc., Madison, WI) for the qualitative detection of varicella-zoster virus (VZV) and differentiation of wild-type and Oka vaccine VZV. The analytical sensitivity of the VZV Invader Plus reagents is at 10 copies per reaction. A total of 174 skin and mucous swab specimens were used to validate the assay's performance. The sensitivity and specificity were 98.3% and 98.1%, respectively, in comparison to a PCR-EIA assay. A perfect 100% agreement was obtained when VZV wild-type and vaccine differentiation was performed on 54 VZV-positive swab specimens against an allele-specific FRET real-time assay. The Invader Plus method provides another reliable tool for qualitative detection of VZV and differentiation of wild-type and vaccine virus. (c) 2007 Elsevier B.V. All rights reserved.
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