Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 10, Pages 1599-1603Publisher
SPRINGER
DOI: 10.1007/s10529-007-9425-3
Keywords
Coomassie Brilliant Blue; mass spectrometry; plant proteomics; protein staining method; two-dimensional electrophoresis
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A more sensitive and convenient Coomassie Brilliant Blue (CBB) staining method for visualizing proteins was developed. Compared with the modifications include the supplement of 10% (v/v) methanol into the fixing solution, an increase of an additional sensitization step and CBB raised from 0.1 to 0.125%. The improved method can detect proteins at nanogram level. The improved method is more sensitive than Blue Silver and more convenient than the Silver protocol. Mass spectrometry results confirmed that it is suitable for subsequent proteomic research.
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