Journal
FEBS LETTERS
Volume 581, Issue 24, Pages 4616-4620Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.08.052
Keywords
ABC transporter; cholesterol; cholesterol effluxl; ABCG5; ABCG8
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The ATP binding cassette transporters ABCG5 and ABCG8 are indispensable for hepatobiliary cholesterol transport. In this study, we investigated the specificity of the heterodimer for cholesterol acceptors. Dog gallbladder epithelial cells were mono- or double-transfected with lentiviral mouse Abcg5 and Abcg8 vectors. Double-transfected cells showed increased efflux to different bile salt (BS) species, while mono-transfected cells did not show enhanced efflux. The efflux was initiated at micellar concentrations and addition of phosphatidylcholine increased efflux. Cholesterol secretion was highly BS dependent, whereas other cholesterol acceptors such as ApoAI, HDL or methyl-beta-cyclodextrin did not elicit Abcg5/g8 dependent cholesterol secretion. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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