Journal
FEBS LETTERS
Volume 581, Issue 24, Pages 4697-4703Publisher
WILEY
DOI: 10.1016/j.febslet.2007.08.074
Keywords
reggie/flotillin; actin; membrane cytoskeleton; membrane microdomain
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The reggies/flotillins are oligomeric scaffolding proteins for membrane microdomains. We show here that reggie-1/flotillin-2 microdomains are organized along cortical F-actin in several cell types. Interaction with F-actin is mediated by the SPFH domain as shown by in vivo co-localization and in vitro binding experiments. Reggie-1/flotillin-2 microdomains form independent of actin, but disruption or stabilization of the actin cytoskeleton modulate the lateral mobility of reggie-1/flotillin-2 as shown by FRAP. Furthermore, reggie/flotillin microdomains can efficiently be immobilized by actin polymerisation, while exchange of reggie-1/flotillin-2 molecules between microdomains is enhanced by actin disruption as shown by tracking of individual microdomains using TIRF microscopy. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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