Journal
FEBS LETTERS
Volume 581, Issue 24, Pages 4761-4766Publisher
WILEY
DOI: 10.1016/j.febslet.2007.08.076
Keywords
APOBEC3G; DNA cytosine deamination; DNA editing; hypermutation
Funding
- NIAID NIH HHS [AI073167, AI064046, R21 AI073167-01A1, R01 AI064046-02, R01 AI064046, R01 AI064046-03, R01 AI064046-01A1, R21 AI073167, R56 AI064046, R33 AI073167, R37 AI064046] Funding Source: Medline
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APOBEC3G is a single-strand DNA cytosine deaminase capable of blocking retrovirus and retrotransposon replication. APOBEC3G has two conserved zinc-coordinating motifs but only one is required for catalysis. Here, deletion analyses revealed that the minimal catalytic domain consists of residues 198-384. Size exclusion assays indicated that this protein is monomeric. Many (31169) alanine substitution derivatives of APOBEC3G198-384 retained significant to full levels of activity. These data corroborated an APOBEC2-based structural model for the catalytic domain of APOBEC3G indicating that most non-essential residues are solvent accessible and most essential residues cluster within the protein core. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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