Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 858, Issue 1-2, Pages 199-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.08.024
Keywords
aesulin; reversed-phase HPLC; pharmacokinetics; metronidazole; plasma
Ask authors/readers for more resources
A sensitive and reproducible high performance liquid chromatography method with UV detection was described for the determination of aesculin in rat plasma. After deproteinization by methanol using metronidazole as internal standard (I.S.), solutes were evaporated to dryness at 40 degrees C under a gentle stream of nitrogen. The residue was reconstituted in 100 mu l of mobile phase and a volume of 20 mu l was injected into the HPLC for analysis. Solutes were separated on a Diamonsil C-18 column (250 mm x 4.6 mm i.d., 5 mu m particle size, Dikma) protected by a ODS guard column (10 mm x 4.0 mm i.d., 5 mu m particle size), using acetonitrile-0.1% triethylamine solution (adjusted to pH 3.0 using phosphoric acid) (10:90, v/v) as mobile phase (flow-rate 1.0 ml/min), and wavelength of the UV detector was set at 338 nm. No interference from any endogenous substances was observed during the elution of aesculin and internal standard (I.S., metronidazole). The retention times for I.S and aesculin were 10.4 and 12.4 min, respectively. The limit of quantification was evaluated to be 57.4 ng/ml and the limit of detection was 24.0 ng/ml. The method was used in the study of pharmacokinetics of aesculin after intraperitoneal injection (i.p.) administration in rats. (C) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available