Journal
JOURNAL OF EXPERIMENTAL BIOLOGY
Volume 210, Issue 20, Pages 3636-3643Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jeb.008334
Keywords
chitin; chitin synthase; Manduca sexta; chymotrypsin-like protease; midgut; peritrophic matrix
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The chitin portion of the peritrophic matrix in the midgut of the tobacco hornworm, Manduca sexta, is produced by chitin synthase 2 (CHS2), a transmembrane family II glycosyltransferase, located at the apical tips of brush border microvilli. To look for proteins that potentially interact with CHS2, we performed yeast two-hybrid screening, identifying a novel chymotrypsin-like protease (CTLP1) that binds to the extracellular carboxyterminal domain of CHS2. The occurrence of this interaction in vivo is supported by co-localization and co-immunoprecipitation data. Based on our findings we propose that chitin synthesis is controlled by an intestinal proteolytic signalling cascade linking chitin synthase activity to the nutritional state of the larvae.
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