Journal
ANAEROBE
Volume 18, Issue 1, Pages 55-61Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.anaerobe.2011.09.007
Keywords
Resistant starch; Clostridium butyricum; Eubacterium rectale; Short chain fatty acid
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Funding
- Indonesia Danone Institute Foundation
- Indonesia Danone Institute
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Resistant starch type 3 (RS3) is retrograded starch which is not digested by human starch degrading enzyme, and will thus undergo bacterial degradation in the colon. The main fermentation products are the Short Chain Fatty Acid (SCFA): acetate, propionate and butyrate. SCFA has significant benefit impact on the metabolism of the host. The objectives of this research were to study the SCFA profile produced by colonic butyrate producing bacteria grown in medium containing RS3. RS3 was made from sago or rice starch treated with amylase, pullulanase and the combination of amylase and pullulanase. Fermentation study was performed by using Clostridium butyricum BCC 82571 or Eubacterium rectale DSM 17629, which has been identified as capable of degradation of starch residue and also regarded as beneficial bacteria. Experimental result revealed that enzyme hydrolysis of retrograded sago or rice starch was beneficial to RS formation. RS3 derived from sago contained higher RS (31-38%) than those derived from rice starch (21-26%). This study indicated that C. butyricum BCC B2571 produced acetate, propionate and butyrate at molar ratio of 1.8: 1 : 1, when the medium was supplemented with RSSA at concentration 1%. In the medium containing similar substrate. E. rectale DSM 17629 produced acetate, propionate and butyrate at molar ratio of 1.7: 1: 1.2. High levels of acetate, propionate and butyrate at molar ratio of 1.8 : 1: 1.1 was also produced by E. rectale DSM 17629 in medium supplemented with RSSP at concentration 1%. The results showed that both bacteria responded differently to the RS3 supplementation. Such result provided insight into the possibility of designing RS3 as prebiotic with featured regarding SCFA released in the human colon with potential health implication. (C) 2011 Elsevier Ltd. All rights reserved.
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