Journal
ANAEROBE
Volume 16, Issue 4, Pages 380-386Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.anaerobe.2010.06.006
Keywords
Bifidobacterium animalis; Selenium-contained protein; Selenium; Antioxidant activity
Categories
Funding
- China National 863 Program [2008AA10Z324, 2006AA10Z343]
- Beijing Natural Science Foundation [5072025]
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Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl ((OH)-O-center dot) and superoxide radicals (O-center dot(2)-) assays, in which the last two assays were measured by electron spin (r)esonance (ESR). There were two kinds of B. animalis 01 proteins in this study, the regular B. animalis 01 protein (Pro-CM) and the B. animalis 01 selenium-contained protein (Pro-Se). Both Pro-CM and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the B. animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of B. animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of B. animalis 01 protein. (C) 2010 Elsevier Ltd. All rights reserved.
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