Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 43, Issue 8, Pages 1099-1108Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2007.07.014
Keywords
-
Funding
- NCRR NIH HHS [P41 RR010888-090113, P41 RR010888-080113, P41 RR010888-090178, P41 RR010888-080178, P41 RR010888-107081, P41 RR010888, P41 RR010888-107136] Funding Source: Medline
- NHLBI NIH HHS [R01 HL031607-23, R01 HL055620, R01 HL031607-20, P01 HL068758-01A10003, R01 HL031607-16, R01 HL031607, R01 HL31607, P01 HL055854-040003, P01 HL055854-030003, R01 HL055620-07, R01 HL031607-21A1, R01 HL055620-09, P01 HL068758, R01 HL031607-22, P01 HL081587, P01 HL055854-050003, P01 HL 68758, R01 HL055620-05, R01 HL055620-06, R01 HL031607-19, P01 HL081738, R01 HL031607-24, P01 HL055854-020003, R01 HL055620-04, R01 HL055620-08, N01HV28178, R01 HL068812-01A1, R01 HL031607-17, R01 HL031607-18, N01-HV-28178, P01 HL081587-010004] Funding Source: Medline
- NIA NIH HHS [R01 AG027080, R01AG27080, R01 AG027080-01, R01 AG027080-02, R01 AG027080-03] Funding Source: Medline
Ask authors/readers for more resources
Cysteine thiol modifications are increasingly recognized to occur under both physiological and pathophysiological conditions, making their accurate detection, identification, and quantification of growing importance. Among free cysteines, the bulk of modifications occurs on a subset of cysteines that are more reactive. These exist as thiolate anions at physiological pH because of their surrounding electrostatic environment. Reagents with iodoacetamide-active groups can be used to selectively label these reactive thiols with a high degree of selectivity. Thiol adducts can be detected by the failure to label with iodoacetamide or other reagents; restoration of labeling by specific reducing agents (e.g., ascorbate or glutaredoxin) can be used to detect reversible S-nitroso and S-glutathione adducts. These adducts also may be detected with radiolabels and antibodies. S-Glutathiolation in response to physiological stimuli may be detected in cells and tissues with glutathione ester labeled with biotin. Mass spectrometry can identify thiol modifications with precision, and with isotope-coded affinity tags, used to quantify modification of specific thiols. Combinations of these methods increase sensitivity and specificity, and enable quantification and precise identification of thiol modifications that occur under physiological and pathological conditions. 2007 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available