Journal
ANAEROBE
Volume 14, Issue 4, Pages 219-223Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.anaerobe.2008.05.001
Keywords
Bifidobacterium adolescentis; polymerase chain reaction; 16S-23S internal transcribed spacers
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Funding
- Council of Agriculture, Taipei, Taiwan
- [96-Agri-1.2.1-Mu-U1 (26)]
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Effective methods for the identification and enumeration of lactic acid producing bacteria (LAB) cells are important for the quality control and assurance of probiotic products. In this study, we designed a polymerase chain reaction (PCR) primer set from the sequence in 16S-23S internal transcribed spacer (ITS) region and used it for the specific detection of Bifidobacterium adolescentis, one of the Bifidobacterium species used in probiotics. Specificity of the PCR primers, i.e., bits-1/bits-2, was assured by assay strains of B. adolescentis, other Bifidobacterium species, and strains of non-Bifidobacterium spp. Coupled with the use of a known primer set specific for Bifidobacterium species, Bifidobacterium strains and B. adolescentis could be identified from LAB strains in fermented dairy products and human fecal samples. (C) 2008 Elsevier Ltd. All rights reserved.
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