Journal
OPTICS EXPRESS
Volume 15, Issue 21, Pages 14184-14193Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.15.014184
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The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences. (c) 2007 Optical Society of America.
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