Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 377, Issue -, Pages 385-392Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2014.07.032
Keywords
Ultraviolet photodissociation; Collision induced dissociation; Glycopeptide
Funding
- NIH [R21GM099028]
- Robert A. Welch Foundation [F1155]
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A comparison of the fragmentation pathways of both protonated and deprotonated O-inked glycopeptides from fetuin and kappa-casein obtained upon collision induced dissociation (CID) and 193 nm ultraviolet photodissociation (UVPD) in a linear ion trap is presented. A strategy using nonspecific pronase digestion, zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) solid phase extraction (SPE) enrichment, and nano-liquid chromatography (nano-LC) is employed. UVPD of deprotonated glycopeptides generally produced the greatest array of fragment ions, thus affording the most diagnostic information about both glycan structure and peptide sequence. In addition, UVPD generated unique fragment ion such as Y-type ions arising from cleavage at the N-terminus of proline. CID and UVPD of protonated glycopeptides produced fragment ions solely from glycan cleavages. (C) 2014 Elsevier B.V. All rights reserved.
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