Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1169, Issue 1-2, Pages 235-238Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.08.085
Keywords
green tea polyphenols; (-)-epigallocatechin gallate; EGCG; agarose gel media; mixed-mode adsorption chromatography
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(-)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum mobile phase compositions regarding purity, recovery and separation time of 78/22 (v/v). Maximum practical sample loading was 100 mg GTP per run (corresponding to 4.2 mg/ml Superose). An EGCG purity of 99% with recoveries in the range 60-65% was achieved in one step directly from the crude GTP extract. Full column regeneration was obtained using solvents in the following order: 0.5 M NaOH, distilled water and 30% acetic acid. (c) 2007 Elsevier B.V. All rights reserved.
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