Biomass production of anoectochilus formosanus hayata in a Bioreactor system

We investigated the factors that affect biomass production from Anoectochilus formosanus in a bioreactor system. Those factors included inoculum size, initial sucrose concentration, media supplements, photosynthetic photon flux density (PPFD), and culturing methods. An inoculum size of 8 g L-1 was most suitable for shoot proliferation; biomass accumulation was optimized when the medium was supplemented with 3% sucrose compared with sucrose-free media or those containing concentrations of 6% or 9%. This accumulation also was enhanced under a PPFD of 50 mu mol m(-2) s(-1). Likewise, the addition of coconut water (50 mL L-1) plus activated charcoal (0.5 mg L-1) to our Hyponex medium proved most beneficial. Comparative studies among three bioreactor systems - continuous immersion, raft (net), and temporary immersion (the ebb and flood system) - revealed that shoot proliferation and biomass accumulation were more efficient when culturing was performed under continuous immersion.