Journal
CELL MOTILITY AND THE CYTOSKELETON
Volume 64, Issue 11, Pages 822-832Publisher
WILEY-LISS
DOI: 10.1002/cm.20226
Keywords
wound healing; cytokinesis; actomyosin; F-actin imaging
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Funding
- NIGMS NIH HHS [R01 GM052932-09, P50 GM066050, GM52932, GM66050, R01 GM052932] Funding Source: Medline
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Actin filaments (F-actin) are protein polymers that undergo rapid assembly and disassembly and control an enormous variety of cellular processes ranging from force production to regulation of signal transduction. Consequently, imaging of F-actin has become an increasingly important goal for biologists seeking to understand how cells and tissues function. However, most of the available means for imaging F-actin in living cells suffer from one or more biological or experimental shortcomings. Here we describe fluorescent F-actin probes based on the calponin homology domain of utrophin (Utr-CH), which binds F-actin without stabilizing it in vitro. We show that these probes faithfully report the distribution of F-actin in living and fixed cells, distinguish between stable and dynamic F-actin, and have no obvious effects on processes that depend critically on the balance of actin assembly and disassembly.
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