Journal
AMYOTROPHIC LATERAL SCLEROSIS
Volume 10, Issue 4, Pages 229-U8Publisher
INFORMA HEALTHCARE
DOI: 10.1080/17482960902871009
Keywords
Cerebrospinal fluid; metabolomics; amyotrophic lateral sclerosis; chemometrics; CO2 evaporization
Categories
Funding
- Swedish Brain Power Consortium
- Hallstens Research Foundation
- Swedish Brain Research Foundation
- Swedish Medical Society
- Bjorklund Foundation
- ALS Research
- Swedish Association for the Neurologically Disabled
Ask authors/readers for more resources
There is a need for biomarkers for early diagnosis, development and evaluation of treatment efficacy in amyotrophic lateral sclerosis (ALS). We aimed to investigate if pre-analytical factors induce artefacts in metabolomic data of cerebrospinal fluid (CSF) from patients with ALS. CSF from 16 patients was studied using a statistical experimental design protocol with the following parameters: storage temperature (-80 degrees C/-20 degrees C), type of collection tube (polypropylene/polystyrene), and time delay from collecting to freezing (0, 10, 30, 90, 150 min). Gas chromatography-mass spectrometry was used to analyse CSF from 12 of the patients while CSF from one patient was analysed with nuclear magnetic resonance spectroscopy. The extent of CO2 evaporization from CSF collected in tubes of different sizes at different temperatures and with/without lid were studied in three addtional patients. We found that alterations in storage temperature affect the metabolite composition of CSF more than any other studied pre-analytical parameter. CO2 evaporization may induce artefacts in the metabolome by increasing the pH. In conclusion, minimization of evaluated artefacts can be obtained by collecting the CSF directly into tubes with tightly sealed lids in N-2(1) and after freezing transfer of the tubes to -80 degrees C to minimize evaporation of CO2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available