Journal
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
Volume 19, Issue 3, Pages 133-137Publisher
INFORMA HEALTHCARE
DOI: 10.3109/13506129.2012.700287
Keywords
beta-Amyloid; A beta; Alzheimer's disease; Peptide synthesis; SPPS
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Funding
- Korea Institute of Science and Technology [2V02950, 2E22310]
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Feasible and reproducible synthesis of full-length A beta peptides has been one of the major challenges in Alzheimer's disease research. By using dimethyl sulfoxide as an anti-aggregation solvent and as an agent to promote double-coupling of two phenylalanine that frequently experience residual deletion, we developed a reliable manual Fmoc solid phase peptide synthesis procedure to produce biologically active A beta in large quantities at relatively high purity. The amyloidogenic activity of the synthesized A beta was confirmed via thioflavin T assay, transmission electron microscopic analysis and electrophoresis.
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