Journal
CURRENT MICROBIOLOGY
Volume 55, Issue 5, Pages 389-395Publisher
SPRINGER
DOI: 10.1007/s00284-007-9008-5
Keywords
twitching motility; PilJ; type IV pili; pseudomonas
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Funding
- NIGMS NIH HHS [GM61318-01] Funding Source: Medline
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Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles.
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