Journal
JOURNAL OF SEPARATION SCIENCE
Volume 30, Issue 16, Pages 2656-2665Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200700228
Keywords
Apis mellifera; Brazilian green propolis; HPLC; phenolic compounds
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A sensitive and reliable RP-HPLC method was developed using a C18 CLC-ODS (M) 4.6 x 250 mm(2) column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83 - 533.33 mu g/mL.
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