Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the-constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.