Journal
JOURNAL OF FLUORESCENCE
Volume 17, Issue 6, Pages 811-816Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-007-0259-0
Keywords
single-molecule fluorescence; metal-enhanced fluorescence; silver nanoparticle; lifetime; plasmonic-controlled fluorescence; DNA; radiative decay engineering
Funding
- NHGRI NIH HHS [HG 02655, R01 HG002655-04A1, R01 HG002655-05, R01 HG002655, R01 HG002655-06, R01 HG002655-03] Funding Source: Medline
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In this short paper, we reported the enhanced fluorescence from a single fluorophore bound to a 50nm silver nanoparticle. We found that on average the Cy5 molecules bound to metal nanoparticles are approximately 15-fold brighter than that of free dyes, and that single molecule lifetimes are shorter as compared to free fluorophores. The increased emission rate is primarily the result of local plasmon enhancement. These results demonstrate that the use of fluorophore-metal interactions can increase the brightness and photostability of fluorophores for single molecule detection.
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