Journal
AMINO ACIDS
Volume 47, Issue 10, Pages 2199-2204Publisher
SPRINGER WIEN
DOI: 10.1007/s00726-014-1781-4
Keywords
Apoptosis; Autophagy; Cytotoxicity; Enterotoxigenic Escherichia coli; IPEC-1; Methionine
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Funding
- National Natural Science Foundation of China [31110103909, 31272217, 31101729, 31201813]
- National Science and Technology Support Program [2012BAD39B03]
- Nanjing Branch Academy of Chinese Academy of Science
- Jiangxi Province Cooperation Project
- Hunan Project [2013RS4065, 2012GK4066]
- Guandong project [2012B091100210]
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Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without l-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy.
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